Clonality:

Monoclonal

Synonyms:

DNA repair protein XRCC4; X-ray repair cross-complementing protein 4

Host:

Mouse

Reactivity:

Human

Applications:

WB, IHC, IP

Background:

Mouse monoclonal antibody to XRCC4

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of human XRCC4. The exact sequence is proprietary.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/2000), IH (1/200 - 1/500), IP (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of XRCC4 expression in Hela (A), 293T (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of XRCC4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

Immunoprecipitation of XRCC4 from 0.5mg Hela whole cell extract lysate, using anti-XRCC4 Antibody.

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.