Clonality:

Monoclonal

Synonyms:

SWS2; EF-hand domain-containing protein D1; EF-hand domain-containing protein 1; Swiprosin-2

Host:

Mouse

Reactivity:

Human, Mouse, Rat

Applications:

WB, IF/IC

Background:

Mouse monoclonal antibody to Swiprosin-2

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of human Swiprosin-2. The exact sequence is proprietary.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/2000), IF/IC (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of Swiprosin-2 expression in mouse spleen (A), rat spleen (B) whole cell lysates.

IHC description:

IF/ICC description:

Immunofluorescent analysis of Swiprosin-2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.