Clonality:

Monoclonal

Synonyms:

API4; IAP4; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin

Host:

Mouse

Reactivity:

Human, Rat

Applications:

WB, IHC

Background:

Mouse monoclonal antibody to Survivin

Immunogen:

Recombinant protein corresponding to human Survivin.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/2000), IH (1/200 - 1/500)

Conjugation:

WB description:

Western blot analysis of Survivin expression in Hela (A), 293 (B), PC12 (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of Survivin staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.