Clonality:

Monoclonal

Synonyms:

APRF; Signal transducer and activator of transcription 3; Acute-phase response factor

Host:

Mouse

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC

Background:

Mouse monoclonal antibody to STAT3

Immunogen:

Recombinant protein corresponding to human STAT3.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/2000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of STAT3 expression in Hela (A), NIH3T3 (B), PC12 (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of STAT3 staining in human colon cancer,mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.