Clonality:

Monoclonal

Synonyms:

GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha

Host:

Mouse

Reactivity:

Mouse, Rat

Applications:

WB, IHC

Background:

Mouse monoclonal antibody to PI3K p85 alpha

Immunogen:

Recombinant protein corresponding to mouse PI3K p85 alpha.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/2000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of PI3K p85 alpha expression in NIH3T3 (A), rat liver (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of PI3K p85 alpha staining in rat liver,mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.