Clonality:

Monoclonal

Synonyms:

Proliferating cell nuclear antigen; PCNA; Cyclin

Host:

Mouse

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC

Background:

Mouse monoclonal antibody to PCNA

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of human PCNA. The exact sequence is proprietary.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/2000 - 1/5000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of PCNA expression in Hela (A), 293T (B), NIH3T3 (C), rat brain (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of PCNA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.