Clonality:

Monoclonal

Synonyms:

NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Host:

Mouse

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC, IP

Background:

Mouse monoclonal antibody to NF-kappaB p65

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of human NF-kappaB p65. The exact sequence is proprietary.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/3000), IH (1/100 - 1/200), IP (1/50 - 1/200)

Conjugation:

WB description:

Western blot analysis of NF-kappaB p65 expression in Hela (A), 293T (B), HepG (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of NF-kappaB p65 staining in mouse hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

Immunoprecipitation of NF-kappaB p65 from 0.5mg Hela whole cell extract lysate, using anti-NF-kappaB p65 Antibody.

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.