Clonality:

Monoclonal

Synonyms:

DNA excision repair protein ERCC-1

Host:

Mouse

Reactivity:

Human

Applications:

WB, IHC

Background:

Mouse monoclonal antibody to ERCC1

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of human ERCC1. The exact sequence is proprietary.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/2000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of ERCC1 expression in Hela (A), HepG2 (B), 293T (C), Jurkat (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of ERCC1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.