Clonality:

Monoclonal

Synonyms:

Nitric oxide synthase endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII

Host:

Mouse

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC

Background:

Mouse monoclonal antibody to eNOS

Immunogen:

Recombinant protein corresponding to human eNOS.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/2000), IH (1/200 - 1/500)

Conjugation:

WB description:

Western blot analysis of eNOS expression in rat heart (A) whole cell lysates.

IHC description:

Immunohistochemical analysis of eNOS staining in mouse colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.