Clonality:

Monoclonal

Synonyms:

ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Host:

Mouse

Reactivity:

Human

Applications:

WB, IF/IC, IP

Background:

Mouse monoclonal antibody to EGFR

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of human EGFR. The exact sequence is proprietary.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200), IP (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of EGFR expression in A431 (A), Hela (B) whole cell lysates.

IHC description:

IF/ICC description:

Immunofluorescent analysis of EGFR staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

Immunoprecipitation of EGFR from 0.5mg Hela whole cell extract lysate, using anti-EGFR Antibody.

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.