Clonality:

Monoclonal

Synonyms:

Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1

Host:

Mouse

Reactivity:

Human, Mouse

Applications:

WB, IHC

Background:

Mouse monoclonal antibody to CREB

Immunogen:

Recombinant protein corresponding to human CREB.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of CREB expression in A431 (A), NIH3T3 (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of CREB staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.