Clonality:

Monoclonal

Synonyms:

PD1; Programmed cell death protein 1; Protein PD-1; hPD-1; CD279

Host:

Mouse

Reactivity:

Human

Applications:

IHC

Background:

Mouse monoclonal antibody to CD279

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within human CD279. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

IH (1/100 - 1/300)

Conjugation:

WB description:

Immunohistochemical analysis of CD279 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IHC description:

Immunohistochemical analysis of CD279 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.