Clonality:

Monoclonal

Synonyms:

MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6

Host:

Mouse

Reactivity:

Human, Mouse, Rat

Applications:

WB, IF/IC, IP

Background:

Mouse monoclonal antibody to Caspase 9

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of human Caspase 9. The exact sequence is proprietary.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/3000), IP (1/50 - 1/200)

Conjugation:

WB description:

Western blot analysis of Caspase 9 expression in Hela (A), Jurkat (B), HepG2 (C), mouse heart (D), rat heart (E) whole cell lysates.

IHC description:

IF/ICC description:

Immunofluorescent analysis of Caspase 9 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

Immunoprecipitation of Caspase 9 from 0.5mg Hela whole cell extract lysate, using anti-Caspase 9 Antibody.

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.