Clonality:

Monoclonal

Synonyms:

TUBB5; Tubulin beta chain; Tubulin beta-5 chain

Host:

Mouse

Reactivity:

Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Sheep, Insect, Yeast

Applications:

WB, IHC

Background:

Mouse monoclonal antibody to Beta-tubulin-HRP labeled

Immunogen:

Recombinant protein corresponding to human Beta-tubulin.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/20000 - 1/600000), IH (1/1000 - 1/2000)

Conjugation:

HRP

WB description:

Western blot analysis of Beta-tubulin-HRP labeled expression in Hela (A) whole cell lysates.

IHC description:

Immunohistochemical analysis of Beta-tubulin-HRP labeled staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.