Clonality:

Monoclonal

Synonyms:

TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III

Host:

Mouse

Reactivity:

Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit

Applications:

WB, IHC, IP

Background:

Mouse monoclonal antibody to Beta-tubulin

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of human Beta-tubulin. The exact sequence is proprietary.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 0.2% BSA, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/3000), IH (1/50 - 1/100), IP (1/50 - 1/100)

Conjugation:

WB description:

Western blot analysis of Beta-tubulin expression in Hela (A), mouse kidney (B), rat heart (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of Beta-tubulin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

Immunoprecipitation of Beta-tubulin from 0.5mg mouse brain whole cell extract lysate, using anti-Beta-tubulin Antibody.

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.