Clonality:

Monoclonal

Synonyms:

HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin

Host:

Mouse

Reactivity:

Human

Applications:

WB, IHC

Background:

Mouse monoclonal antibody to Alpha-fetoglobulin

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of human Alpha-fetoglobulin. The exact sequence is proprietary.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/2000), IH (1/200 - 1/500)

Conjugation:

WB description:

Western blot analysis of Alpha-fetoglobulin expression in HepG2 (A) whole cell lysates.

IHC description:

Immunohistochemical analysis of Alpha-fetoglobulin staining in human hepatocancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.