Clonality:

Polyclonal

Synonyms:

DNA topoisomerase 2-beta; DNA topoisomerase II, beta isozyme

Host:

Rabbit

Reactivity:

Human, Dog, Monkey, Pig

Applications:

WB, IHC, IF/IC

Background:

Rabbit polyclonal antibody to Topoisomerase 2 beta

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Topoisomerase 2 beta. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Conjugation:

WB description:

Western blot analysis of Topoisomerase 2 beta expression in K562 (A), Jurkat (B), MCF7 (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of Topoisomerase 2 beta staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

Immunofluorescent analysis of Topoisomerase 2 beta staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.