Clonality:

Polyclonal

Synonyms:

TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

Host:

Rabbit

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to TNF alpha

Immunogen:

Recombinant protein corresponding to human TNF alpha.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/2000), IH (1/50 - 1/200)

Conjugation:

WB description:

Western blot analysis of TNF alpha expression in HepG2 LPS-treated (A) whole cell lysates.

IHC description:

Immunohistochemical analysis of TNF alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.