Clonality:

Polyclonal

Synonyms:

TCF1; Transcription factor 7; TCF-7; T-cell-specific transcription factor 1; T-cell factor 1; TCF-1

Host:

Rabbit

Reactivity:

Human, Bovine

Applications:

WB, IHC, IP

Background:

Rabbit polyclonal antibody to TCF7

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human TCF7. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Conjugation:

WB description:

Western blot analysis of TCF7 expression in Jurkat (A), HCT29 (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of TCF7 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.