Clonality:

Polyclonal

Synonyms:

TAK1; Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1; TGF-beta-activated kinase 1

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Bovine, Pig, Zebrafish

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to TAK1 (pT187)

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TAK1. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of TAK1 (pT187) expression in A431 (A), HeLa (B), mouse liver (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of TAK1 (pT187) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.