Rabbit anti-TACSTD2 Antibody YLD7566
Specifications
| 50ul / 100ul price = 100ul |
Clonality:
Polyclonal
Synonyms:
GA733-1; M1S1; TROP2; Tumor-associated calcium signal transducer 2; Cell surface glycoprotein Trop-2; Membrane component chromosome 1 surface marker 1; Pancreatic carcinoma marker protein GA733-1
Host:
Rabbit
Reactivity:
Human, Mouse, Rat, Pig
Applications:
WB, IHC, IF/IC
Background:
Rabbit polyclonal antibody to TACSTD2
Immunogen:
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TACSTD2. The exact sequence is proprietary.
Purification method:
The antibody was purified by immunogen affinity chromatography.
Concentration:
1mg/mL
Buffer:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Conjugation:
WB description:
Western blot analysis of TACSTD2 expression in human prostate (A), NIH3T3 (B) whole cell lysates.
IHC description:
Immunohistochemical analysis of TACSTD2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description:
Immunofluorescent analysis of TACSTD2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
IP description:
FC description:
ChIP description
Storage:
Store at -20℃. Avoid repeated freeze / thaw cycles.