Clonality:

Polyclonal

Synonyms:

Signal transducer and activator of transcription 5B

Host:

Rabbit

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to STAT5B

Immunogen:

Recombinant protein corresponding to human STAT5B.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/2000), IH (1/200 - 1/500)

Conjugation:

WB description:

Western blot analysis of STAT5B expression in K562 (A), Hela (B), NIH3T3 (C), mouse heart (D), PC12 (E), rat heart (F) whole cell lysates.

IHC description:

Immunohistochemical analysis of STAT5B staining in human spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.