Clonality:

Polyclonal

Synonyms:

ERLIN1; C10orf69; KE04; KEO4; SPFH1; Erlin-1; Endoplasmic reticulum lipid raft-associated protein 1; Protein KE04; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 1; SPFH domain-containing protein 1; ERLIN2; C8orf2; SPFH2; Erlin-2; Endoplasmic reticulum lipid raft-associated protein 2; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2; SPFH domain-containing protein 2

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Bovine, Zebrafish

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to SPFH1

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SPFH1. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Conjugation:

WB description:

Western blot analysis of SPFH1 expression in HepG2 (A), NIH3T3 (B), Hela (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of SPFH1 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.