Clonality:

Polyclonal

Synonyms:

MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Chicken, Monkey, Pig, Sheep

Applications:

WB, IF/IC, IP

Background:

Rabbit polyclonal antibody to SMAD3 (pS204)

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SMAD3. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Conjugation:

WB description:

Western blot analysis of SMAD3 (pS204) expression in HeLa UV-treated (A), SP2/0 UV-treated (B), H9C2 UV-treated (C) whole cell lysates.

IHC description:

IF/ICC description:

Immunofluorescent analysis of SMAD3 (pS204) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.