Clonality:

Polyclonal

Synonyms:

Paired amphipathic helix protein Sin3a; Histone deacetylase complex subunit Sin3a; Transcriptional corepressor Sin3a

Host:

Rabbit

Reactivity:

Human, Mouse, Rat

Applications:

WB, IF/IC, IP, ChIP

Background:

Rabbit polyclonal antibody to SIN3A

Immunogen:

Recombinant full length protein of human SIN3A

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200), IP (1/50 - 1/200), ChIP (1/20 - 1/100)

Conjugation:

WB description:

Western blot analysis of SIN3A expression in Hela (A), MCF7 (B), COS7 (C) whole cell lysates.

IHC description:

IF/ICC description:

Immunofluorescent analysis of SIN3A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.