Clonality:

Polyclonal

Synonyms:

PPP1R38; SH2A; SH2 domain-containing protein 4A; Protein SH(2)A; Protein phosphatase 1 regulatory subunit 38

Host:

Rabbit

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to SH2D4A

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SH2D4A. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of SH2D4A expression in HEK293T (A), Raw264.7 (B), PC12 (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of SH2D4A staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.