Clonality:

Polyclonal

Synonyms:

CCAAT/enhancer-binding protein epsilon; C/EBP epsilon

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Sheep

Applications:

WB, IHC, IF/IC

Background:

Rabbit polyclonal antibody to C/EBP epsilon (pT74)

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human C/EBP epsilon. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/100 - 1/500)

Conjugation:

WB description:

Western blot analysis of C/EBP epsilon (pT74) expression in Hela (A), mouse liver (B), Raw264.7 (C), rat liver (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of C/EBP epsilon (pT74) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

Immunofluorescent analysis of C/EBP epsilon (pT74) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.