Clonality:

Polyclonal

Synonyms:

FACD; FANCD1; Breast cancer type 2 susceptibility protein; Fanconi anemia group D1 protein

Host:

Rabbit

Reactivity:

Human, Rat, Monkey

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to BRCA2

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human BRCA2. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of BRCA2 expression in MDAMB231 (A), MCF7 (B), rat lung (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of BRCA2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.