Clonality:

Polyclonal

Synonyms:

BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Chicken, Zebrafish

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to B-RAF

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human B-RAF. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of B-RAF expression in HL60 (A), HeLa (B), NIH3T3 (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of B-RAF staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.