Clonality:

Polyclonal

Synonyms:

APG4D; AUTL4; Cysteine protease ATG4D; AUT-like 4 cysteine endopeptidase; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D

Host:

Rabbit

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC, IP

Background:

Rabbit polyclonal antibody to ATG4D

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATG4D. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/100)

Conjugation:

WB description:

Western blot analysis of ATG4D expression in Hela (A), A549 (B), PC12 (C), CT26 (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of ATG4D staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.