Clonality:

Polyclonal

Synonyms:

Aspartate--tRNA ligase, mitochondrial; Aspartyl-tRNA synthetase; AspRS

Host:

Rabbit

Reactivity:

Human

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to AspRS

Immunogen:

Recombinant full length protein of human AspRS

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Conjugation:

WB description:

Western blot analysis of AspRS expression in NCIH460 (A), U251 (B), MCF7 (C), BT474 (D), mouse liver (E), mouse heart (F) whole cell lysates.

IHC description:

Immunohistochemical analysis of AspRS staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.