Clonality:

Polyclonal

Synonyms:

DP2.5; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Monkey, Pig

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to APC

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human APC. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of APC expression in HeLa (A), A431 (B), NIH3T3 (C), H9C2 (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of APC staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.