Clonality:

Polyclonal

Synonyms:

AMPK; 5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Bovine, Pig

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to AMPK beta 1 (pS182)

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AMPK beta 1. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of AMPK beta 1 (pS182) expression in A431 (A), NIH3T3 (B), PC12 (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of AMPK beta 1 (pS182) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.