Clonality:

Polyclonal

Synonyms:

Aminoacylase-1; ACY-1; N-acyl-L-amino-acid amidohydrolase

Host:

Rabbit

Reactivity:

Human

Applications:

WB, IHC, IF/IC

Background:

Rabbit polyclonal antibody to Aminoacylase 1

Immunogen:

Recombinant full length protein of human Aminoacylase 1

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/10 - 1/100)

Conjugation:

WB description:

Western blot analysis of Aminoacylase 1 expression in A431 (A), Jurdat (B), mouse brain (C), mouse liver (D), rat kidney (E) whole cell lysates.

IHC description:

Immunohistochemical analysis of Aminoacylase 1 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

Immunofluorescent analysis of Aminoacylase 1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.