Clonality:

Polyclonal

Synonyms:

RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Chicken, Dog

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to AKT2 (pS474)

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human AKT2 (pS474). The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of AKT2 (pS474) expression in Hela (A), mouse brain (B), PC12 UV-treated (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of AKT2 (pS474) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.