Clonality:

Polyclonal

Synonyms:

AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT3; PKBG; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Bovine, Chicken, Zebrafish

Applications:

WB, IF/IC, IP

Background:

Rabbit polyclonal antibody to AKT (pT308)

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AKT. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Conjugation:

WB description:

Western blot analysis of AKT (pT308) expression in HeLa colchicine-treated (A), HL60 (B), NIH3T3 (C), SP2/0 colchicine-treated (D), PC12 colchicine-treated (E) whole cell lysates.

IHC description:

IF/ICC description:

Immunofluorescent analysis of AKT (pT308) staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.