Clonality:

Polyclonal

Synonyms:

Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1

Host:

Rabbit

Reactivity:

Mouse, Rat

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to ACTN1

Immunogen:

Recombinant protein corresponding to mouse ACTN1.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/2000), IH (1/200 - 1/500)

Conjugation:

WB description:

Western blot analysis of ACTN1 expression in NIH3T3 (A), PC12 (B), rat heart (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of ACTN1 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.