Clonality:

Polyclonal

Synonyms:

C6orf2; C6orf4; C6orf5; C6orf6; Adapter protein CIKS; Connection to IKK and SAPK/JNK; Nuclear factor NF-kappa-B activator 1; ACT1; TRAF3-interacting protein 2

Host:

Rabbit

Reactivity:

Human, Mouse

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to ACT1

Immunogen:

Recombinant full length protein of human ACT1

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Conjugation:

WB description:

Western blot analysis of ACT1 expression in MCF7 (A), mouse kidney (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of ACT1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.