Clonality:

Polyclonal

Synonyms:

Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Bovine, Pig

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to 4EBP1 (pT37)

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human 4EBP1 (pT37). The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of 4EBP1 (pT37) expression in HEK293T insulin-treated (A), NIH3T3 insulin-treated (B), PC12 insulin-treated (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of 4EBP1 (pT37) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.