Clonality:

Polyclonal

Synonyms:

HME1; 14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin

Host:

Rabbit

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC, IF/IC

Background:

Rabbit polyclonal antibody to 14-3-3 sigma

Immunogen:

Recombinant full length protein of human 14-3-3 sigma

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Conjugation:

WB description:

Western blot analysis of 14-3-3 sigma expression in HT29 (A), MCF7 (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of 14-3-3 sigma staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

Immunofluorescent analysis of 14-3-3 sigma staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.