Background:

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.

Clonality:

monoclonal

Classification:

Primary antibody

Alternative names:

CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4

Specificity:

Human, Mouse, Rat

Host:

Mouse

Isotype:

IgG2a

Immunogen:

Cytochrome c

Purification:

Protein A+G purification

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

12 kDa

Form:

liquid

Applications:

ELISA, WB, IHC

Recommended dilution:

WB: 1:5000-1:50000; IHC: 1:20-1:800

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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