Background:

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1(MVCD1). VEGFA has 13 isoforms with MW 16kd to 45kd. Some isoforms has homodimer form, such as VEGFA189(38kd), VEFGA110(34kd). VEGFA is a 34kd to 42kd, dimeric, disulfide-bound glycoprotein. VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids(PMID:15602010). This antibody can recognize all the isoforms of VEFGA.

Clonality:

monoclonal

Classification:

Primary antibody

Alternative names:

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

Specificity:

Human

Host:

Mouse

Isotype:

IgG2b

Immunogen:

vascular endothelial growth factor A

Purification:

protein A+G purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

45 kDa

Form:

liquid

Applications:

ELISA, WB, IHC

Recommended dilution:

WB: 1:1000-1:4000; IHC: 1:50-1:500

Storage:

0.01MPBS pH 7.4,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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