Background:

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha and beta tubulins have been widely used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

Clonality:

monoclonal

Classification:

Primary antibody

Alternative names:

Beta tubulin, MC1R, TUBB, Tubulin-beta

Specificity:

Human, Mouse, Rat

Host:

Mouse

Isotype:

IgG1

Immunogen:

tubulin, beta 3

Purification:

Protein A+G purification

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

50-55 kDa

Form:

liquid

Applications:

ELISA, WB, IHC, IF, IP

Recommended dilution:

WB: 1:2000-1:20000; IF: 1:20-1:200; IP: 1:500-1:5000; IHC: 1:20-1:1000

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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