Background:

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein.

Clonality:

monoclonal

Classification:

Primary antibody

Alternative names:

Glutathione S-Transferase, GST, gst tag

Specificity:

Recombinant protein

Host:

Mouse

Isotype:

IgG2a

Immunogen:

Recombinant protein

Purification:

Protein A+G purification

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

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Form:

liquid

Applications:

ELISA, WB, IP

Recommended dilution:

WB: 1:5000-1:50000; IP: 1:5000-1:20000

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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