Background:

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules(By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate(G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT(gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs(such as ceruplasmin) and suppresses their translation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues.

Clonality:

monoclonal

Classification:

Primary antibody

Alternative names:

G3PD, GAPD, GAPDH

Specificity:

Human, Mouse, Rat, Zebrafish

Host:

Mouse

Isotype:

IgG1

Immunogen:

glyceraldehyde-3-phosphate dehydrogenase

Purification:

Protein A+G purification

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

36 kDa

Form:

liquid

Applications:

ELISA, IHC, WB, IF, FC, IP

Recommended dilution:

WB: 1:5000-1:20000; IP: 1:3000-1:20000; IHC: 1:50-1:500; IF: 1:20-1:200

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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