Background:

Rabbit polyclonal antibody to GUF1

Alternative names:

Translation factor GUF1 mitochondrial; Elongation factor 4 homolog; EF-4; GTPase GUF1; Ribosomal back-translocase

Clonality:

Polyclonal

Species reactivity:

H

Host:

Rabbit

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GUF1. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IH

Recommended dilution:

WB (1/500 - 1/1000), IH (1/50 - 1/200)

Storage:

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB description:

Western blot analysis of GUF1 expression in SHSY5Y (A) whole cell lysates.

IHC description:

Immunohistochemical analysis of GUF1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com