Background:

Rabbit polyclonal antibody to Cytokeratin 18

Alternative names:

CYK18; Keratin type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18

Clonality:

Polyclonal

Species reactivity:

H, M, R

Host:

Rabbit

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Cytokeratin 18. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IH, IF/IC

Recommended dilution:

WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)

Storage:

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB description:

Western blot analysis of Cytokeratin 18 expression in HCT116 (A), LO2 (B), SGC7901 (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of Cytokeratin 18 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

Immunofluorescent analysis of Cytokeratin 18 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com