Background:

Rabbit polyclonal antibody to Leptin

Alternative names:

OB; OBS; Leptin; Obese protein; Obesity factor

Clonality:

Polyclonal

Species reactivity:

H, M

Host:

Rabbit

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Leptin. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IH

Recommended dilution:

WB (1/500 - 1/1000), IH (1/50 - 1/200)

Storage:

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB description:

Western blot analysis of Leptin expression in LO2 (A), HCT116 (B), MCF7 (C), A549 (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of Leptin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com