Alternative Name(s):

4; 43 kDa inositol polyphosphate 5-phophatase; 5-trisphosphate 5-phosphatase; 5PTase; CTCL tumor antigen HD-CL-02; DKFZp434A1721; I5P1; I5P1_HUMAN; Inositol polyphosphate 5 phosphatase; inositol polyphosphate-5-phosphatase A; inositol trisphosphate-5-phosphatase; INPP5A; INPP5A inositol polyphosphate-5-phosphatase; InsP3 5-phosphatase; MGC116947; MGC116949; Type I inositol-1; type I inositol-1,4,5-trisphosphate 5-phophatase

Species: (Organism)

Homo sapiens (Human)

Gene Names:

INPP5A

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

MAGKAAAPGTAVLLVTANVGSLFDDPENLQKNWLREFYQVVHTHKPHFMALHCQEFGGKNYEASMSHVDKFVKELLSSDAMKEYNRARVYLDENYKSQEHFTALGSFYFLHESLKNIYQFDFKAKKYRKVAGKEIYSDTLESTPMLEKEKFPQDYFPECKWSRKGFIRTRWCIADCAFDLVNIHLFHDASNLVAWETSPSVYSGIRHKALGYVLDRIIDQRFEKVSYFVFGDFNFRLDSKSVVETLCTKATMQTVRAADTNEVVKLIFRESDNDRKVMLQLEKKLFDYFNQEVFRDNNGTALLEFDKELSVFKDRLYELDISFPPSYPYSEDARQGEQYMNTRCPAWCDRILMSPSAKELVLRSESEEKVVTYDHIGPNVCMGDHKPVFLAFRIMPGAGKPHAHVHKCC

Expression Region:

1-409aa

Subcellular Location:

Membrane, Lipid-anchor

Tissue Specificity:

Brain; high level in Purkinje cells.

Protein Length:

Full Length

Pathway:

Phosphatidylinositolsignalingsystem

Mol. Weight:

63.5 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Signal Transduction

Function:

Major isoenzyme hydrolyzing the calcium-mobilizing second messenger Ins(1,4,5)P3, this is a signal-terminating reaction.

Involvement in disease:

Relevance:

Major isoenzyme hydrolyzing the calcium-mobilizing second messenger Ins(1,4,5)P3, this is a signal-terminating reaction.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Inositol 1,4,5-trisphosphate 5-phosphatase type I family

Reference:

Cloning and expression of human brain type I inositol 1,4,5-trisphosphate 5-phosphatase. High levels of mRNA in cerebellar Purkinje cells.de Smedt F., Verjans B., Mailleux P., Erneux C.FEBS Lett. 347:69-72(1994)