Host:

E.coli

Tag:

His-tag

AA Sequence:

ACCACCCCTGAACCGTGCGAACTGGATGATGAAGACTTCCGCTGCGTGTGTAACTTCAGTGAACCGCAGCCGGATTGGAGCGAAGCCTTCCAGTGCGTTAGTGCAGTTGAAGTGGAAATTCATGCAGGCGGCCTGAATCTGGAACCGTTCCTGAAACGCGTTGATGCAGATGCAGATCCGCGCCAGTATGCCGATACCGTTAAAGCCCTGCGTGTGCGTCGTCTGACCGTGGGTGCAGCACAGGTGCCGGCACAGCTGCTGGTTGGTGCACTGCGTGTTCTGGCCTATAGTCGCCTGAAAGAACTGACCCTGGAAGATCTGAAAATTACCGGTACCATGCCGCCGCTGCCGCTGGAAGCCACCGGTCTTGCACTGAGCAGCCTGCGTCTGCGTAATGTGAGCTGGGCCACCGGTCGCAGCTGGCTGGCAGAACTGCAGCAGTGGCTGAAACCGGGTCTGAAAGTTCTGAGCATTGCCCAGGCACATAGCCCGGCATTCAGTTGCGAACAGGTTCGCGCCTTCCCGGCACTGACCAGTCTGGATCTGAGTGATAATCCGGGTCTGGGCGAACGCGGTCTGATGGCCGCACTGTGTCCGCATAAATTCCCGGCAATTCAGAATCTGGCACTGCGTAATACCGGTATGGAAACACCTACCGGTGTGTGTGCAGCCCTGGCCGCCGCTGGTGTGCAGCCTCATAGCCTGGATCTGTCACATAATAGCCTGCGCGCCACCGTTAATCCGAGCGCCCCGCGTTGCATGTGGAGCAGCGCACTGAATAGCCTGAATCTGAGCTTCGCAGGTCTGGAACAGGTTCCGAAAGGCCTGCCGGCAAAACTGCGTGTTCTTGATCTGAGCTGTAATCGCCTGAATCGCGCACCGCAGCCGGACGAACTGCCGGAAGTGGATAATCTGACCCTGGATGGTAATCCGTTCCTGGTGCCGGGCACCGCACTGCCTCATGAAGGCAGCATGAAT

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~40kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

CD14 is a leucine-rich repeat-containing pattern recognition receptor with expression largely restricted to the monocyte/macrophage cell lineage. Research studies have shown that CD14 is a bacterial lipopolysaccharide (LPS) binding glycoprotein, expressed as either a GPI-linked membrane protein or a soluble plasma protein. LPS induces an upregulation of GPI-linked CD14 expression, which facilitates TLR4 signaling and macrophage activation in response to bacterial infection. The 61D3 antibody is widely used to identify cells of the monocyte/macrophage lineage.

Note:

For research use only, not for use in diagnostic procedure.